Isolation of Cellulolytic Bacteria from Intestine of Termites and Their Utility in Saccharification
👇Download Article👇 https://www.ijert.org/isolation-of-cellulolytic-bacteria-from-intestine-of-termites-and-their-utility-in-saccharification-and-fermentation-of-lignocellulosic-biomass IJERTV9IS100042 Isolation of Cellulolytic Bacteria from Intestine of Termites and Their Utility in Saccharification and Fermentation of Lignocellulosic Biomass Divya Kakara , Dr. Ramesh Malothu , E. Laxmi Narayana Among the various sources (organisms) of cellulolytic enzymes, termites are one of the potential sources from which the cellulolytic bacteria can be isolated from its gut. These cellulolytic bacteria produce the cellulolytic enzymes which are used for maximum break down of cellulose, lingo-cellulose and hemicelluloses into glucose molecules and this glucose is next converted to ethanol by fermentation using co-cultured Saccharomyces cerevisiae. The isolated and screened bacteria in the Congo-red agar media is subjected to Hydrolysis capacity test, which is observed a 7-7.2 range. The process variables like pH, temperature and volume are optimized for maximum growth of cellulolytic bacteria. Flask level fermentation after five days incubation of 10% cellulose taken as the substrate under optimized conditions yielded an average of 33.6 % ethanol as measured using potassium dichromate method. 2.5g/l of crude samples (Sugarcane bagasse and corn cobs) were subjected to pre-treatment with 4% H2SO4, for optimum cellulose, 100gm of pre-treated samples of sugarcane bagasse and corncobs yielded 74ml and 73.5ml of ethanol at 96 hours incubation, when cocultured with Saccharomyces cerevisiae.
👇Download Article👇 https://www.ijert.org/isolation-of-cellulolytic-bacteria-from-intestine-of-termites-and-their-utility-in-saccharification-and-fermentation-of-lignocellulosic-biomass IJERTV9IS100042 Isolation of Cellulolytic Bacteria from Intestine of Termites and Their Utility in Saccharification and Fermentation of Lignocellulosic Biomass Divya Kakara , Dr. Ramesh Malothu , E. Laxmi Narayana Among the various sources (organisms) of cellulolytic enzymes, termites are one of the potential sources from which the cellulolytic bacteria can be isolated from its gut. These cellulolytic bacteria produce the cellulolytic enzymes which are used for maximum break down of cellulose, lingo-cellulose and hemicelluloses into glucose molecules and this glucose is next converted to ethanol by fermentation using co-cultured Saccharomyces cerevisiae. The isolated and screened bacteria in the Congo-red agar media is subjected to Hydrolysis capacity test, which is observed a 7-7.2 range. The process variables like pH, temperature and volume are optimized for maximum growth of cellulolytic bacteria. Flask level fermentation after five days incubation of 10% cellulose taken as the substrate under optimized conditions yielded an average of 33.6 % ethanol as measured using potassium dichromate method. 2.5g/l of crude samples (Sugarcane bagasse and corn cobs) were subjected to pre-treatment with 4% H2SO4, for optimum cellulose, 100gm of pre-treated samples of sugarcane bagasse and corncobs yielded 74ml and 73.5ml of ethanol at 96 hours incubation, when cocultured with Saccharomyces cerevisiae.