Gel electrophoresis: faint bands

For future reference, it may help to check the dilution factor of your ladder if you encounter similar problems. Even if it’s prepared as instructed, high concentration can sometimes cause the camera to focus too much on the ladder's brightness, impacting image quality. By running a few dilution tests to find the ideal balance, you can achieve a clear electrophoresis profile without needing to adjust the PCR setup. https://www.youtube.com/watch?v=tItX0HzfwcY